Single-Molecule Studies of Small Molecule Drugs through Nanopore Detection

During the last decades, nanopore single-molecule analysis has shown great potential in detection of DNA [1-3], RNA [4], proteins [5- 8], polymers [9], organic molecules [10-11], and chemical reactions [12-13], and has been further extended to high-throughput nucleic acid sequencing. The detection of drug interactions [14] is crucial to evaluate the effect and safety of drugs, and it is also an indispensable part in the process of drug development and clinic treatment [15]. Over the past few years, the nanopore worked as a new platform for the discovery of drug interactions. Nanopore sensor, with the advantages of simple operation, speed and portability, can allow single molecule to pass through.