A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor

Staphylococcus aureus is one of the major foodborne pathogens. Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial. Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor. The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus. For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan. The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements. To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.