Wheat low molecular weight glutenin subunits-structrual relationship ot other gluten proteins analyzed using specific antibodies

The immunological homologies of low molecular weight glutenin subunits (LMW-GS) were compared with the other major wheat gluten polypeptides, high molecular weight glutenin subunits (HMW-GS) and gliadins. Conventional one-dimensional polyacrylamide gel electrophoretic (PAGE) methods were used as well as a two-step, one-dimensional sodium dodecyl sulfate-PAGE technique, together with immunoblotting and enzyme-immunoassay methods