Biochip Analyzer as an Equipment Component in “Lab-on-Chip” Technology

The possibilities of quantitative analysis of weak signals from cells of biochip arrays fixed on a substrate, the fluorescence of which is comparable in intensity to the fluorescence of the substrate, were investigated using a specially designed wide-field digital microscope, a highly sensitive biochip analyzer. In the spectral range of cyanine dye Cy5 (λ ex = 645 nm, λ em = 665 nm), the relative levels of intrinsic fluorescence of the surfaces of a number of materials that can serve as substrates for biochip matrices used in medical diagnostics were evaluated. It was shown that the analyzer on a substrate of black polybutylene terephthalate (black crastin) allows detecting the fluorescence of 3 and more Sy5 dye molecules/µm 2 on its surface.