化学
检出限
荧光
点击化学
叠氮化物
核酸
环加成
生物相容性
DNA
组合化学
炔烃
杂交探针
纳米技术
色谱法
生物化学
有机化学
催化作用
物理
量子力学
材料科学
作者
Min Wang,Yang Zhao,Xuewen He,Ben Zhong Tang,Hong Liu,Yu Zhang,Lin Han
出处
期刊:Talanta
[Elsevier]
日期:2023-07-01
卷期号:259: 124562-124562
被引量:2
标识
DOI:10.1016/j.talanta.2023.124562
摘要
A fluorescence assay was developed based on aggregation-induced emission for circulating tumor (ctDNA) rapid detection. AIE-based probe (TPE-DNA) was constructed via strain-promoted azide-alkyne cycloaddition reaction between the azide-functionalized TPE-N3 and the dibenzocyclooctyne functionalized DNA. AIE-based assay displayed significant fluorescence signal enhancement for the ctDNA targets. The experimental results were verified and explained based on density functional theory simulations. As a proof-of-concept, the ctDNA as the target was detected to determine the feasibility of the assay. The detection could be done within 10 min with the limit of detection of 39 pM and good selectivity without the assistance of enzyme or nano-materials. Compared with traditional click reaction, the assay has advantages of high efficiency and good biocompatibility. It is promising in rapid diagnostic of cancer marker nucleic acid with further efforts to develop intelligent miniaturized equipment.
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