The anterior retrosplenial cortex encodes event-related information and the posterior retrosplenial cortex encodes context-related information during memory formation

The retrosplenial cortex (RSC) is extensively interconnected with the dorsal hippocampus and has several important roles in learning and memory. Recent work has demonstrated that certain types of context-dependent learning are selectively impaired when the posterior, but not the anterior, region of the RSC is damaged, suggesting that the role of the RSC in memory formation may not be uniform along its rostro-caudal axis. The current experiments tested the idea that the anterior and posterior portions of the rat RSC contribute to different aspects of memory formation. We first confirmed that brief optogenetic inhibition of either the anterior or posterior RSC resulted in decreased local cellular activity as indexed by immediate early gene zif268 expression and that this decrease was restricted to the target region within RSC. We then found that silencing the anterior or posterior RSC during trace fear training trials had different effects on memory: While inhibiting neural activity in the anterior RSC had a selective impact on behavior evoked by the auditory CS, inhibition of the posterior RSC selectively impaired memory for the context in which training was conducted. These results contribute to a growing literature that supports functionally distinct roles in learning and memory for subregions of the RSC.