安培法
检出限
化学
计时安培法
电极
适体
循环伏安法
肌钙蛋白I
介电谱
胶体金
分析化学(期刊)
纳米技术
电化学
色谱法
纳米颗粒
材料科学
心肌梗塞
精神科
生物
物理化学
心理学
遗传学
作者
Hang-Hyun Jo,Jin Her,Hee‐Hyun Lee,Yoon‐Bo Shim,Changill Ban
出处
期刊:Talanta
[Elsevier]
日期:2017-04-01
卷期号:165: 442-448
被引量:98
标识
DOI:10.1016/j.talanta.2016.12.091
摘要
In this study, we developed a sandwich aptamer-based screen-printed carbon electrode (SPCE) using chronoamperometry for the detection of cardiac troponin I (cTnI), one of the promising biomarkers for acute myocardial infarction (AMI). Disposable three-electrode SPCEs were manufactured using a screen printer, and various modifications such as electrodeposition of gold nanoparticles and electropolymerization of conductive polymers were performed. From the bare electrode to the aptamer-immobilized SPCE, all processes were monitored and analyzed via various techniques such as cyclic voltammetry, electrochemical impedance spectroscopy, and X-ray photoelectron spectroscopy. The quantification of cTnI was conducted based on amperometric signals from the catalytic reaction between hydrazine and H2O2. The fabricated aptasensor in a buffer, as well as in a serum-added solution, exhibited great analytical performance with a dynamic range of 1-100 pM (0.024-2.4ng/mL) and a detection limit of 1.0 pM (24pg/mL), which is lower than the existing cutoff values (40-700pg/mL). Furthermore, the developed sensor showed high sensitivity to cTnI over other proteins. It is anticipated that this potable SPCE aptasensor for cTnI will become an innovative diagnostic tool for AMI.
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