Mouse C2C12 Myoblasts as a Model for Studying Piezo1 Mechanosensitive Channels in Skeletal Muscle

Abstract Immortalized mouse C2C12 myoblasts are an important model for studying the physiology and pathophysiology of skeletal muscle. Since muscle tissue function is inextricably linked to mechanical processes, investigating the activity of various mechanosensory molecules and signaling cascades is a crucial task. Mechanically gated cation channels Piezo1 play a key role in cellular mechanotransduction and mechano-dependent responses in both non-excitable and excitable cells and tissues. Elucidating the role of Piezo1 and the mechanisms of its regulation in C2C12 cells is an important direction in myology, yet remaining in its infancy. In this review, we compile experimental evidence on Piezo1 function and discuss possible signaling pathways and specific features of Piezo1 regulation in C2C12 cells. Identifying the patterns governing Piezo1 activity and associated cellular signaling processes in C2C12 cells may reveal new aspects of Piezo1 involvement that are important not only for fundamental and applied myology but also for other areas of mechanobiology.