Research on the ultrasonic-assisted extraction, purification, structural characterization, and anti- glycolipid metabolic disorder (anti-GLMD) activity and mechanism of polysaccharide from Dryopteridis crassirhizomatis Rhizoma

Dryopteridis crassirhizomatis Rhizoma, the traditional Chinese medicinal herb commonly known as "Mianma Guanzhong", botanically originating from the dried rhizome of Dryopteris crassirhizoma Nakai, exhibits extensive pharmacological activities. To further elaborate on its medicinal potential, a homogeneous polysaccharide, designated as DCP-1-2, was ultrasonic-assisted extracted and purified from the dried rhizomes of Dryopteris crassirhizoma. Subsequently, comprehensive structural elucidation of DCP-1-2 was conducted using a combination of advanced analytical techniques, including HPLC, SERS, methylation analysis, and NMR spectroscopy. Notably, systematic in vitro assessments were performed to evaluate two key biological activities of DCP-1-2: its antioxidant capacity and its inhibitory effects on key enzymes implicated in glucose and lipid metabolism. To this end, as a critical crosslink between glucose and lipid metabolic pathways, AGEs were employed to establish a high-fat cell model. This cell model was then utilized to investigate the anti-GLMD activity of DCP-1-2 and to decipher the underlying molecular mechanisms. Collectively, these findings lay a solid experimental foundation for the potential application of Dryopteris crassirhizoma-derived polysaccharides in the prevention and management of GLMD.