生物
微生物学
系统发育树
分枝杆菌
痰
拉伤
基因组DNA
细菌
支气管肺泡灌洗
脂肪酸
基因
遗传学
肺
肺结核
病理
哲学
解剖
医学
生物化学
语言学
作者
Hanako Fukano,Yuko Kazumi,Nobuya Sakagami,Nagatoshi Fujiwara,Manabu Ato,Satoshi Mitarai,Yoshihiko Hoshino
标识
DOI:10.1099/ijsem.0.005917
摘要
Scotochromogenic slow-growing mycobacteria were isolated from the sputum or bronchoalveolar lavage fluid of 12 patients in Japan. From a comparison of the whole-genome sequences, the representative strain IWGMT90018-18076T and the unknown strains obtained from the patients were found to represent a novel species related to the Mycobacterium gordonae complex. The average nucleotide identity values of IWGMT90018-18076T with Mycobacterium vicinigordonae, Mycobacterium paragordonae and M. gordonae were 86.7, 82.5 and 82.2 %, respectively. The genome size of the representative strain IWGMT90018-18076T was approximately 6.3 Mbp, and the genomic DNA G+C content was 67.1 %. The major fatty acid methyl esters were C16 : 0 (37.71 %), C18 : 1ω9c (29.5 %) and C16 : 1ω7c (10.32 %). In this study, we performed phylogenetic analyses, physiological and biochemical characteristic tests, drug susceptibility tests and fatty acid profiling of the clinical isolates. On the basis of the results obtained, we propose that the unknown clinical isolates represent a novel species, 'Mycobacterium kiyosense sp. nov,' with the type strain being IWGMT90018-18076T (=JCM 34837T =KCTC 49725T).
科研通智能强力驱动
Strongly Powered by AbleSci AI