纳米探针
小RNA
胞外囊泡
细胞外小泡
纳米线
生物标志物
纳米技术
原位
微泡
计算生物学
DNA
材料科学
化学
生物
分子生物学
细胞生物学
纳米颗粒
基因
生物化学
有机化学
作者
Ye Zhang,Yuan Wu,Shihua Luo,Chao Yang,Guangzhi Zhong,Guoni Huang,Xiaohe Zhang,Bo Li,Chunchen Liu,Li Ling,Xiaohui Yan,Lei Zheng,Bo Situ
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-03-21
卷期号:7 (4): 1075-1085
被引量:19
标识
DOI:10.1021/acssensors.1c02717
摘要
Extracellular vesicle-associated miRNAs (EV-miRNAs) are emerging as a new type of noninvasive biomarker for disease diagnosis. Their relatively low abundance, however, makes accurate detection challenging. Here, we designed a DNA nanowire guided-catalyzed hairpin assembly (NgCHA) nanoprobe for profiling EV-miRNAs. NgCHA showed high penetrability to EVs, which allowed rapid delivery of the probes into EVs. In the presence of targeted miRNAs within EVs, a fluorescent signal could be generated and amplified by confining the catalytic hairpin assembly system within the nanowires, thus greatly enhancing the analytical sensitivity. We showed that EV-miRNAs from various cell lines could be accurately quantified by NgCHA in situ. By using a four-EV-miRNA panel, this platform can identify patients with breast cancer at an early stage with 95.2% sensitivity and 86.7% specificity. Its applications for risk assessment as well as cancer type prediction were also successfully demonstrated. This platform is sensitive, low-cost, and simple compared with current methods. It may thus serve as a promising tool for the noninvasive diagnosis and monitoring of cancers and other diseases through EV-miRNA profiling.
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