Study on enzymatic activity and lipase catalysis by lipase high-yield strain

Abstract In this paper, microorganism for high lipase productivity was screened for immobilization in biodiesel synthesis. Strains with high lipase productivity were analyzed at nucleic acid level with special universal primes. In addition, reaction course, theory of lipase adsorption, lipase specificity on substrate, kinetics, and the application of enzymatic synthesis in biodiesel were investigated. The tests of lipase productivity, lipase purification, lipase immobilization, and lipase application in biodiesel were carried out indicating that lipase from the above micro-organisms was able to catalyze the transesterification reaction of rapeseed oil with moderate oleic aicd content.