生物
干细胞
电池类型
转录组
中肠
祖细胞
细胞生物学
肠内分泌细胞
细胞
旁分泌信号
计算生物学
基因表达
遗传学
基因
激素
内分泌系统
内分泌学
植物
受体
幼虫
作者
Ruei-Jiun Hung,Yanhui Hu,Rory Kirchner,Yifang Liu,Chiwei Xu,Aram Comjean,Sudhir Gopal Tattikota,Fangge Li,Wei Song,Shannan Ho Sui,Norbert Perrimon
标识
DOI:10.1073/pnas.1916820117
摘要
Studies of the adult Drosophila midgut have led to many insights in our understanding of cell-type diversity, stem cell regeneration, tissue homeostasis, and cell fate decision. Advances in single-cell RNA sequencing provide opportunities to identify new cell types and molecular features. We used single-cell RNA sequencing to characterize the transcriptome of midgut epithelial cells and identified 22 distinct clusters representing intestinal stem cells, enteroblasts, enteroendocrine cells (EEs), and enterocytes. This unbiased approach recovered most of the known intestinal stem cells/enteroblast and EE markers, highlighting the high quality of the dataset, and led to insights on intestinal stem cell biology, cell type-specific organelle features, the roles of new transcription factors in progenitors and regional variation along the gut, 5 additional EE gut hormones, EE hormonal expression diversity, and paracrine function of EEs. To facilitate mining of this rich dataset, we provide a web-based resource for visualization of gene expression in single cells. Altogether, our study provides a comprehensive resource for addressing functions of genes in the midgut epithelium.
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