草木犀根瘤菌
渗透压
耐旱性
生物
中华根瘤菌属
苜蓿
玉米素
萎蔫
渗透调节剂
根际细菌
农学
接种
根瘤菌科
植物
园艺
共生
细胞分裂素
脯氨酸
细菌
生物化学
根际
遗传学
生长素
基因
氨基酸
作者
Wei Yu,Li Luo,Xiangyu Qi,Yuman Cao,Jie An,Zongqiang Xie,Tianming Hu,Peizhi Yang
标识
DOI:10.1021/acs.jafc.4c00115
摘要
Plant growth-promoting rhizobacteria have been shown to enhance plant tolerance to drought stress through various mechanisms. However, there is limited research on improving drought resistance in alfalfa by genetically modifying PGPR to produce increased levels of cytokinins. Herein, we employed synthetic biology approaches to engineer two novel strains of Sinorhizobium meliloti capable of overproducing trans-Zeatin and investigated their potential in enhancing drought tolerance in alfalfa. Our results demonstrate that alfalfa plants inoculated with these engineered S. meliloti strains exhibited reduced wilting and yellowing while maintaining higher relative water content under drought conditions. The engineered S. meliloti-induced tZ activated the activity of antioxidant enzymes and the accumulation of osmolytes. Additionally, the increased endogenous tZ content in plants alleviated the impact of drought stress on the alfalfa photosynthetic rate. However, under nondrought conditions, inoculation with the engineered S. meliloti strains had no significant effect on alfalfa biomass and nodule formation.
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