Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53

赖氨酸 生物膜 微生物学 肽聚糖 自愈水凝胶 鲍曼不动杆菌 抗菌剂 多重耐药 化学 伤口愈合 抗生素 噬菌体 细菌 生物 免疫学 生物化学 铜绿假单胞菌 大肠杆菌 有机化学 基因 遗传学
作者
Changchang Li,Raphael Nyaruaba,Xiaowei Zhao,Heng Xue,Yuhong Li,Hang Yang,Hongping Wei
出处
期刊:Viruses [Multidisciplinary Digital Publishing Institute]
卷期号:14 (9): 1956-1956 被引量:3
标识
DOI:10.3390/v14091956
摘要

Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The coupling of lysins with a material that will cover the wound may prove beneficial in both protecting and treating wound infections. Hence, in this study, a Gram-negative lysin, LysP53, was coupled with a thermosensitive hydrogel, poloxamer P407, and its efficacy to treat wound infection was tested. In vitro, the addition of LysP53 to the poloxamer did not affect its thermosensitive characteristics, nor did it affect the hydrogel structure. Moreover, the lysin hydrogel could hydrolyze the peptidoglycan, demonstrating that it may have bactericidal activity. Up to 10.4% of LysP53 was released from the hydrogel gradually within 24 h, which led to a 4-log reduction of stationary phase Acinetobacter baumannii. Lastly, the lysin hydrogel was found safe with no cytotoxic effects observed in cells. Ex vivo, LysP53 hydrogel could inhibit bacterial growth on a pig skin decolonization model, with 3-log differences compared to non-treated groups. Overall, our results suggest that lysin-loaded hydrogels may provide a novel solution to treat wound infections caused by resistant bacteria.

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