Extraction and Enzymatic Properties of β-Glucosidase in Chrysanthemum morifolium

Objective To determine the activity ofβ-glucosidase in Chrysanthemum morifolium,and to study the fundamental enzymatic properties ofβ-glucosidase in Chrysanthemum morifolium.Methods The p-nitrophenylD-glucoside(pNPG)method was used to measure the optimal condition for determination of the activity ofβ-glucosidase in Chrysanthemum morifolium and its enzymatic properties.Results Theβ-glucosidase in Chrysanthemum morifolium had the highest activity when the mixture of 0.2 mL enzyme solution,0.2mL pPNG(20mmol/L),and 0.6mL citric acid-trisodium citrate buffer(pH 4.0)was incubated for 45 min in a 50 ℃ water bath.The fundamental enzymatic properties ofβ-glucosidase in the crude enzyme solution of Chrysanthemum morifolium were preliminarily studied under the above condition.The results showed that the enzymatic activity increased as the temperature increased from 30 ℃to 50 ℃,suggesting a high thermal stability of the enzyme.The relative activity of the enzyme kept higher than 80% at pH3.2-5.4.The enzyme was quite stable during storage at 4 ℃.Conclusion The optimal condition for the activity ofβ-glucosidase in Chrysanthemum morifolium and its enzymatic properties determined in this study provide a basis for the processing principle of Chrysanthemum morifolium.