Effect of PLC-γ1 on cell proliferation induced by platelet-derived growth factor

： Objective To study the effect of PLC-γ1 on cell proliferation induced by platelet-derived growth factor(PDGF). Methods The immortalized fibroblasts genetically deficient in PLC-γ1(PLC-γ1-/-) was used to investigate the role of PLC-γ1 in cell proliferation induced by PDGF, which involves the mobilization of intracellular Ca2+ and DNA synthesis. Results DNA synthesis was increased and intrcellular Ca2+ mobilization detected in both PLC-γ1-/- and PLC-γ1+/+ cells, but in the former, the time consumption of DNA synthesis was prolonged, and the mobilization of Ca2+ attenuated and postponed. Conclusion PLC-γ1 is important in cellular mitogenic signal transduction induced by PDGF, but its deficiency may be compensated through other signal transduction pathways.