Effects of hydroxycamptothecin liposome combined with cisplatin on cell cycle and apoptosis of cisplatin-resistanted human esophageal carcinoma Eca109 cell

Aim: To investigate the effects of hydroxycamptothecin liposome( LHCPT) combined with cisplatin( DDP) on cell cycle and apoptosis of DDP-resistanted human esophageal carcinoma cell Eca109( Eca109 / DDP). Methods: Eca109 / DDP cells were treated with 0,1 mg / L DDP,and then treated with 0. 00,0. 49,0. 98,1. 96,3. 92,7. 84 μg / L LHCPT for 72 h,respectively; MTT method was applicated to detect the cell proliferation. Eca109 / DDP cells were treated with 0,1 mg / L DDP,and then treated with 0. 00,3. 92 μg / L LHCPT for 72 h,respectively; flow cytometry was used to study the cell cycle and apoptosis. Results: Compared with single drug treatment,the growth inhibition effect on Eca109 / DDP cells in LHCPT + DDP group was significant( FLHCPT= 40. 330,FDDP= 641. 010,Finteraction= 20. 330,P 0. 001),especially 3. 92 μg/L LHCPT +1 mg/L DDP. Compared with single drug treatment,3. 92 μg/L LHCPT +1 mg/L DDP could significantly block Eca109 / DDP cells at G0/ G1phase( FLHCPT= 16. 184,FDDP= 33. 660,Finteraction= 15. 100,P 0. 05),and increase the early apoptotic rate,late apoptotic rate and total apoptotic rate( P 0. 05). Conclusion: LHCPT has the potential to be an adjunct to chemotherapy for esophageal carcinoma.