Identification of Homologous Recombination of Lincomycin Biosynthesis Defective Strains

Strain YY1 and YY2 were two genetic recombinant strains derived from S.lincolnensis B48 by homologous exchange and homologous integration, respectively. Compared with B48, the potency of lincomycin of YY2 was much lower, while that of YY1 turned to be zero. The genetic recombination was further identified by PCR and the result showed that two copies of lmbI genes in YY1 were inactivated by homologous exchange, while probably only one copy in YY2 was inactivated by homologous integration and the other remained intact.