C2C12型
辅活化剂
转录因子
心肌细胞
细胞生物学
生物
调节器
LIM域
肌发生
基因
生物化学
锌指
作者
Yuki Takakura,Takayuki Suzuki,Naoki Hirai,Takuro Araki,Masatoshi Ohishi,Hiromi Sato,Naoto Yamaguchi,Hiroyuki Takano,Noritaka Yamaguchi
标识
DOI:10.1016/j.bbrc.2023.05.073
摘要
Vestigial-like family member 3 (VGLL3) is a cofactor for the TEA-domain transcription factor (TEAD) family. Although VGLL3 influences myogenic differentiation, its involvement in slow- and fast-twitch fiber specification remains unknown. In this study, we established a cell line stably overexpressing VGLL3 and analyzed effects of VGLL3 on the myogenic differentiation of murine myoblast C2C12 cells. We found that VGLL3 expression promotes slow-twitch muscle differentiation. Mechanistically, VGLL3 expression induced the expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), a master transcriptional regulator of slow-twitch muscle development. We also found that VGLL3 proteins are degraded by the proteasome, which causes switching of TEAD cofactors from VGLL3 to Yes-associated protein (YAP) and transcriptional coactivator with a PDZ-binding motif (TAZ). These results suggest that the balance between the two kinds of TEAD cofactors VGLL3 and YAP/TAZ controls muscle fiber-type specification.
科研通智能强力驱动
Strongly Powered by AbleSci AI