猪流行性腹泻病毒
病毒学
生物
RNA聚合酶
RNA依赖性RNA聚合酶
核糖核酸
多克隆抗体
病毒复制
病毒
微生物学
分子生物学
基因
抗体
遗传学
作者
Suya Liao,Yijing Chen,Yongrong Yang,Guanhua Wang,Qihang Wang,Jiaxin Liu,Haowen Wu,Qiyuan Luo,Yun Chen
标识
DOI:10.1016/j.jim.2023.113442
摘要
The RNA synthesis of porcine epidemic diarrhea virus (PEDV) is a sophisticated process performed by a multilingual viral replication complex, together with cellular factors. A key enzyme of this replication complex is RNA-dependent RNA polymerase (RdRp). However, there is limited knowledge about PEDV RdRp. In our present study, a polyclonal antibody against RdRp was prepared by using a prokaryotic expression vector pET-28a-RdRp to study the function of PEDV RdRp and provide a tool to investigate PEDV pathogenesis. In addition, the enzyme activity and half-life of PEDV RdRp were investigated. The result showed that the polyclonal antibody against PEDV RdRp was successfully prepared and was able to be used to detect PEDV RdRp by immunofluorescence and western blotting. Additionally, enzyme activity of PEDV RdRp reached nearly 2 pmol/μg/h and the half-life of PEDV RdRp was 5.47 h.
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