Background: Romanowsky stains are universally used for routine staining of peripheral blood smears. Among these Leishman stain is most commonly used in hematology laboratories worldwide. This study was done to stain peripheral blood smears with modified Leishman stain (MLS) on Day 1, Day 5, Day 10 of preparation of the stain and to assess the quality of staining by scoring the stained smears by calculating the Quality Index (QI) and comparing the scores with normal peripheral smear.