Banha-sasim-tang improves gastrointestinal function in loperamide-induced functional dyspepsia mouse model

洛哌丁胺 胃排空 莫沙比利 医学 汉方 内科学 酚红 药理学 运动性 胃肠病学 内分泌学 腹泻 化学 生物 病理 替代医学 色谱法 遗传学
作者
Yoo-Jin Jeon,Jin‐Seok Lee,Yong-Rae Cho,Sung-Bae Lee,Youn‐Jung Kim,Seong‐Soo Roh,Jin‐Yong Joung,Hwa Dong Lee,Sung-Ok Moon,Jung‐Hyo Cho,Chang‐Gue Son
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:238: 111834-111834 被引量:15
标识
DOI:10.1016/j.jep.2019.111834
摘要

Banha-sasim-tang (BST; Hange-shashin-to in Kampo medicine; Banxia xiexin tang in traditional Chinese medicine) is a traditional Chinese harbal medicine that has been commonly used for gastrointestinal disorders. To investigate the pharmacological effects of BST, a standardized herbal drug, on main symptoms of functional dyspepsia including delayed gastric emptying, and underlying mechanisms of action in mouse model. Balb/C mice were pretreated with BST (25, 50, 100 mg/kg, po) or mosapride (3 mg/kg, po) for 3 days, and then treated with loperamide (10 mg/kg, ip) after 19 h fasting. A solution of 0.05% phenol red (500 μL) or 5% charcoal diet (200 μL) was orally administered, followed by scarifying and assessment of gastric emptying or gastro-intestinal motility. C-kit (immunofluorescence), nNOS (western blot) and gastric contraction-related gene expression were examined in stomach tissue. The loperamide injection substantially delayed gastric emptying, while the BST pretreatment significantly attenuated this peristaltic dysfunction, as evidenced by the quantity of stomach-retained phenol red (p < 0.05 or 0.01) and stomach weight (p < 0.05 or 0.01). The BST pretreatment significantly tempered the loperamide-induced inactivation of c-kit and nNOS (p < 0.05 or 0.01) as well as the contraction-related gene expression, such as the 5HT4 receptor (5HT4R), anoctamin-1 (ANO1), ryanodine receptor 3 (RYR3) and smooth muscle myosin light chain kinase (smMLCK). The BST pretreatment also significantly attenuated the alterations in gastro-intestinal motility (p < 0.01). Our results are the first evidence of the prokinetic agent effects of Banha-sasim-tang in a loperamide-induced FD animal model. The underlying mechanisms of action may involve the modulation of peristalsis via activation of the interstitial cells of Cajal and the smooth muscle cells in the stomach.
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