Asiaticoside Alleviates Alzheimer's Disease by Regulating PPP1CC Expression to Suppress Inflammation and Mitochondrial Dysfunction.

OBJECTIVE: Alzheimer's disease (AD) is a neurodegenerative disorder. Asiaticoside (AS), one of the main active components of Centella asiatica, shows therapeutic potential in various diseases, including AD. However, the specific molecular mechanisms by which AS treats AD remain unclear. METHODS: levels. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect interleukin-1β (IL-1β) and IL-6 levels. GeneCards, comparative toxicogenomics database (CTD), and swisstargetprediction databases were used to obtain AD and AS targets. Enrichment analysis and plotting were performed using the clusterProfiler package in R. The simplified molecular input line entry system (SMILES) website was used to obtain the 3D structure of AS. The universal protein resource (UniProt) website was used to obtain the protein structure of protein phosphatase 1 catalytic subunit gamma (PPP1CC). Autodock v4.2.6 was used for molecular docking. RESULTS: -induced HBMECs similar to those exerted by AS, whereas PPP1CC overexpression produced the opposite effects. CONCLUSION: -induced HBMECs, likely through modulating PPP1CC expression.