化学
球虫抑制剂
拉沙酸
色谱法
莫能星
电喷雾电离
电喷雾
质谱法
盐霉素
球虫病
有机化学
抗生素
钙
离子载体
医学
生物化学
兽医学
作者
Konrad Pietruk,Małgorzata Olejnik,Piotr Jedziniak,Teresa Szprengier–Juszkiewicz
标识
DOI:10.1016/j.jpba.2015.03.019
摘要
A multi-residue method has been developed and validated for the simultaneous determination of authorized (decoquinate, diclazuril, halofuginone, lasalocid, maduramicin, monensin, narasin, nicarbazin, robenidine, salinomycin and semduramicin) and non-authorized (amprolium, clopidol, ethopabate and toltrazuril) coccidiostats in animal feed. Feed samples were extracted with basic followed by acidified solution in methanol and, after centrifugation, were injected directly into LC-MS/MS system. Detection was performed in selected reaction monitoring mode with both positive and negative electrospray ionization. The time efficient validation experiment has verified the robustness of a method in different types of feed and on two separate LC-MS/MS instruments. The comparison of different quantification methods demonstrated that, against expectations, the standard addition did not prove better in comparison with matrix-matched calibration curve. Although the sample preparation was very easy, the observed matrix effects were not significant for the most part but they could explain the problems with the quantification of some coccidiostats.
科研通智能强力驱动
Strongly Powered by AbleSci AI