Antioxidant Activities Bioactive Compound of Ethyl Acetate Extracts from rose myrtle Leaves (Rhodomyrtus tomentosa (Ait.) Hassk.)

Abstract One of the plants traditionally used as medicine is rose myrtle (Rhodomyrtus tomentosa (Ait.) Hassk.). Rose myrtle has the potential as an antioxidant because it has secondary metabolites including flavonoids, phenols, terpenoids, tannins, saponins, and steroids. The purpose of this study was to determine the fraction that has antioxidant activity and obtain antioxidant compounds from ethyl acetate extract of rose myrtle leaves. The research methods included extraction by Soxhletasi, fractionation by vacuum liquid chromatography, purification of compounds with gravity columns, and antioxidant activity test using the DPPH method. The results obtained 32% yield of ethyl acetate extract which was then followed by fractionation. The fractionation results obtained 13 fractions. The fractions that have antioxidant activity namely fractions 5-6, 7, and 8. The fraction was continued to purification and obtained by compound E.1, compound E.2 from the essential oil, and compound E3 allegedly rhodomyrtone. The bioactive compound determined by IC 50 value obtained that E1, E2 and E3 that have IC 50 value of 218, 207 and 90 μg/mL, respectively. The bioactive compounds of ethyl acetate extract have strong antioxidant activity in E3 and very weak in E1 and E2 compounds.