显微镜
量子点
分辨率(逻辑)
纳米技术
荧光
荧光显微镜
材料科学
超分辨显微术
超分辨率
光学
计算机科学
物理
人工智能
图像(数学)
作者
Xusan Yang,Karl Zhanghao,Hening Wang,Yujia Liu,Fan Wang,Xu Zhang,Kebin Shi,Juntao Gao,Dayong Jin,Peng Xi
出处
期刊:ACS Photonics
[American Chemical Society]
日期:2016-08-02
卷期号:3 (9): 1611-1618
被引量:53
标识
DOI:10.1021/acsphotonics.6b00178
摘要
Quantum dots (QDs) are well known as bright and photostable inorganic fluorescent probes for microscopy imaging, with many attractive features superior to those found in organic dyes. However, their broadband excitation spectrum and emission blinking property have limited the applicability of QDs in modern super-resolution microscopy techniques. In this work, we systematically investigate practical approaches to overcoming these drawbacks and provide examples of their use across many commercially available super-resolution microscopy systems now accessible to biologists, with examples across the major super-resolution techniques. This work further maps out how QDs can be further engineered to facilitate their applications in the respective super-resolution microscopy techniques.
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