透明质酸
细胞外基质
CD44细胞
化学
整合素
体外
糖蛋白
肿瘤微环境
细胞生物学
基质(化学分析)
跨膜蛋白
生物物理学
生物化学
肿瘤细胞
生物
癌症研究
解剖
细胞
受体
色谱法
作者
Sarbajeet Dutta,Shamik Sen
出处
期刊:Methods in molecular biology
日期:2023-12-02
卷期号:: 131-139
标识
DOI:10.1007/978-1-0716-3589-6_11
摘要
Collagen-I (Col) and hyaluronic acid (HA) are two of the most important extracellular matrix (ECM) components. While collagen serves as the main structural feature of the ECM and is an important ligand for integrin-based adhesions, HA acts as a ligand for the transmembrane glycoprotein CD44. However, most studies on cancer invasion utilize 3D collagen matrices, thereby ignoring the importance of HA–CD44 interactions. Here, we describe the process of fabricating Col–HA gels with varying Col/HA ratios, which can be used to suitably mimic the tumor microenvironment.
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