Optimized prime editing efficiently generates glyphosate-resistant rice plants carrying homozygous TAP-IVS mutation in EPSPS

Prime editing is a novel and universal CRISPR-Cas-derived precise genome-editing technology and has great potentials for applications in basic plant research and crop molecular breeding (Anzalone et al., 2019). Although low efficiency has restrained the original prime editors (PEs) from being used as a routine tool for precise genome editing in plants, an iterative update of the PEs is removing this obstacle (Lin et al., 2021; Xu et al., 2022). Recently, the Liu group reported three optimization strategies for improving prime editing efficiency (Chen et al., 2021; Nelson et al., 2022).