Psychromarinibacter halotolerans gen. nov., sp. nov., isolated from seawater of the Yellow Sea

A Gram-stain-negative, strictly aerobic, motile, non-gliding, oxidase-positive, catalase-positive, rod-shaped bacterium, designated strain YBW34T, was isolated from seawater from the bottom of the Yellow Sea at station H12 (-73m in depth). Growth occurred at 10-45 °C (optimum 28 °C), in the presence of 1-12 % NaCl (w/v, optimum 4 %) and at pH 6.0-8.0 (optimum pH 7.0). The major fatty acids (>10 %) were C18 : 1 ω7c and C16 : 0. The major polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and three unidentified aminolipids. The major respiratory quinone was ubiquinone-10 (Q-10). The DNA G+C content of strain YBW34T was 64.2 mol%. The most closely related species was Tropicimonas isoalkanivorans JCM 14837T with 95.8 % sequence similarity in Alphaproteobacteria. It showed 16S rRNA gene sequence similarities of 93.03-95.49, 93.03-95.49 and 95.31-95.32 % to species of genera Rhodovulum, Lutimaribacter and Oceanicola, respectively. Nevertheless, strain YBW34T formed a distinct lineage in the trees which did not join the genera mentioned above in the phylogenetic dendrogram based on 16S rRNA gene sequences. The phenotypic, chemotaxonomic and phylogenetic data indicated that strain YBW34T represents a novel genus and species, for which the name Psychromarinibacter halotolerans gen. nov., sp. nov. is proposed. The type strain is YBW34T (=JCM 31462T=KCTC 52366T=MCCC 1K03203T).