Collagen-based hydrogels cross-linked via laccase - mediated system incorporated with Fe3+ for wound dressing

Pure fish skin collagen hydrogels as a wound dressing have lower thermodynamic stability than mammalian collagen and usually suffer from poor mechanical properties, weak degradation resistance and insufficient functionalities such as antioxidant and anti-inflammatory properties to meet clinical needs that limit its further application. Here, a silver carp skin collagen hydrogel is successfully constructed via the cross-linking of the laccase-protocatechuic aldehyde (LAC-PAL) and the structure of the hydrogel is further consolidated and strengthened by the interaction of PAL and Fe3+. In this collagen hydrogel system, Fe3+, acting as a second cross-linker, consolidates and enhances the stability of the hydrogel after LAC-PAL cross-linking. This cross-linking method improves the resistance to degradation with a reduction in its degradation rate from 89.45% to 38.66% and endows the hydrogel with antioxidant activity. The in vitro data show that the hydrogel promotes cell proliferation and adhesion exhibiting good biocompatibility. Animal experiments show that the hydrogel contributes to angiogenesis and improves inflammatory response in the early stages of wound healing, resulting in promoting wound healing. Altogether, this newly developed collagen hydrogel is expected to be applied in wound repair as a wound dressing.