DETERMINATION OF EMAMECTIN BENZOATE RESIDUES IN WATER BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

The residue analysis method for determination of emamectin benzoate in water with HPLC-FLD after pre-column derivatization was developed.Emamectin benzoate was extracted with ethyl acetate,and the extract was derivatized with NMIM-CAN(1+1)and TFAA-CAN(1+1)for 20 minutes under the dark.The derivative was detected by HPLC.The results showed that,the recoveries of emamectin benzoate was 78.4%—115.2% and the method coefficient of variation was 3.0%—11.3%,for spiked water samples at the concentration of 0.0001—0.01mg·l-1 emamectin benzoate.The limit of quantification(LOQ)was 0.0036mg·l-1.The residue of emamectin benzoate in the water of Chaohu lake was below the detection limit of this method,while in the water of paddy field was 0.54mg·l-1.