病毒学
接种疫苗
免疫系统
鼻腔给药
抗体
中和抗体
生物
病毒
免疫学
病毒进入
病毒复制
医学
作者
Philipp A. Ilinykh,Sivakumar Periasamy,Kai Huang,Natalia Kuzmina,P. Ramanathan,Michelle Meyer,Chad E. Mire,Ivan Kuzmin,Preeti Bharaj,Jessica R Endsley,Maria Chikina,Stuart C. Sealfon,Steven G. Widen,Mark Endsley,Alexander Bukreyev
出处
期刊:npj vaccines
[Springer Nature]
日期:2022-04-25
卷期号:7 (1)
被引量:5
标识
DOI:10.1038/s41541-022-00471-3
摘要
Respiratory tract vaccination has an advantage of needle-free delivery and induction of mucosal immune response in the portal of SARS-CoV-2 entry. We utilized human parainfluenza virus type 3 vector to generate constructs expressing the full spike (S) protein of SARS-CoV-2, its S1 subunit, or the receptor-binding domain, and tested them in hamsters as single-dose intranasal vaccines. The construct bearing full-length S induced high titers of neutralizing antibodies specific to S protein domains critical to the protein functions. Robust memory T cell responses in the lungs were also induced, which represent an additional barrier to infection and should be less sensitive than the antibody responses to mutations present in SARS-CoV-2 variants. Following SARS-CoV-2 challenge, animals were protected from the disease and detectable viral replication. Vaccination prevented induction of gene pathways associated with inflammation. These results indicate advantages of respiratory vaccination against COVID-19 and inform the design of mucosal SARS-CoV-2 vaccines.
科研通智能强力驱动
Strongly Powered by AbleSci AI