骨保护素
骨桥蛋白
骨钙素
运行x2
碱性磷酸酶
体内
骨整合
内分泌学
激活剂(遗传学)
内科学
受体
材料科学
植入
生物
医学
外科
生物化学
酶
生物技术
作者
Paula Gabriela Faciola Pessôa de Oliveira,Mariana Sales de Melo Soares,Adriana Maria Mariano Silveira e Souza,Mário Taba,Daniela Bazan Palioto,Michel Reis Messora,Bruna Ghiraldini,Felipe Anderson Nunes,Sérgio Luı́s Scombatti de Souza
摘要
This study evaluated the response of a nano-hydroxyapatite coating implant through gene expression analysis (runt-related transcription factor 2 (Runx2), alkaline phosphatase (Alp), osteopontin (Opn), osteocalcin (Oc), receptor activator of nuclear factor-kappa B (Rank), receptor activator of nuclear factor-kappa B ligand (Rank-L), and osteoprotegerin (Opg)). Three-dimensional evaluation (percent bone volume (BV/TV); percent intersection surface (BIC); bone surface/volume ratio (BS/BV); and total porosity (To.Po)) were also analyzed. Mini implants were surgically placed in tibias of both healthy and diabetic rats. The animals were euthanized at 7 and 30 days. Evaluating all factors the relative expression of Rank showed that NANO surface presented the best results at 7 days (diabetic rats). Furthermore the levels of Runx2, Alp, Oc, and Opn suggest an increase in osteoblasts proliferation, especially in early stages of osseointegration. %BIC in healthy and diabetic (7 days) depicted statistically significant differences for NANO group. BV/TV, BS/BV and To.Po demonstrated higher values for NANO group in all evaluated time point and irrespective of systemic condition, but BS/BV 30 days (healthy rat) and 7 and 30 days (diabetic rat). Microtomographic and gene expression analyses have shown the benefits of nano-hydroxyapatite coated implants in promoting new bone formation in diabetic rats.
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