鼠疫耶尔森菌
毒力
生物
微阵列
基因
微生物学
基因表达谱
微阵列分析技术
鼠疫(疾病)
基因表达
抗原
抗体
分子生物学
遗传学
历史
考古
作者
Bei Li,Yafang Tan,Jingyu Guo,Baizhong Cui,Zuyun Wang,Hu Wang,Lei Zhou,Zhaobiao Guo,Ziwen Zhu,Zongmin Du,Ruifu Yang
出处
期刊:Canadian Journal of Microbiology
[Canadian Science Publishing]
日期:2011-04-01
卷期号:57 (4): 287-294
被引量:7
摘要
Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26 °C, and mammalian hosts, which have a body temperature of 37 °C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. pestis was used to evaluate antibody responses in a pooled plague serum that was unadsorbed, adsorbed by Y. pestis cultivated at 26 °C, or adsorbed by Y. pestis cultivated at 26 and 37 °C to identify protein expression changes during the temperature shift. We identified 12 proteins as being expressed at 37 °C but not at 26 °C, or expressed at significantly higher levels at 37 °C than at 26 °C. The antibodies against 7 proteins in the serum adsorbed by Y. pestis cultivated at 26 and 37 °C remained positive, suggesting that they were not expressed on the surface of Y. pestis in LB broth in vitro or specifically expressed in vivo. This study proved that protein microarray and antibody profiling comprise a promising technique for monitoring gene expression at the protein level and for better understanding pathogenicity, to find new vaccine targets against plague.
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