Extraction and Separation of Aucubin from the Leave of Eucommia ulmoides in Aqueous Two-phase System

A new method to extract and separate aucubin from the leave of Eucommia ulmoides Oliv.in aqueous two-phase system formed by macromolecular compound PEG4000 and dextran 40000 was investigated.The effect of phase map of extraction system,the addition amount of sample solution,pH and temperature were investigated systematically.The results showed that the best ATPS was composed of 11% PEG4000 and 8% D40,and addition of sample was 8 g at 60 ℃,pH 7.The highest extraction rate could reach to 66.32% after three times.The content of aucubin in the extract from liquid of aqueous two-phase extraction could rise up to 48.67 % from 8.750%.