Simultaneous determination of psoralen and isopsoralen in Psoralea corylifolia

In order to establish a fast and simple determination method for Psoralea corylifolia, the psoralen and isopsoralen contents were determined in the Psoralea corylifolia samples from different regions. The optimum experimental condition was set up by single-factor and orthogonal design test, and the psoralen andisopsoralen were separated and determined by TLC-UV. The psoralen contents in the samples from different regions were 3.357 ～5.768 mg/g, and isopsoralen contents were 2.235 ～5.354 mg/g. The psoralen and isopsoralen contents in the sample of Guangxi province is highest. The method has high duplication and exactitude.