Effect of black soldier fly protein powder and oil on palatability of dog food and analysis of main aroma substances

The study was to compare palatability of different dog foods by a two-bowl test. Three kinds of dog food were designed, dog food 1 was chicken meal + fish oil chicken oil, dog food 2 was black horsefly protein powder + fish oil and chicken oil, and dog food 3 was chicken powder + black horsefly grease. The volatile aroma compounds of dog food were extracted and analyzed by gas chromatography-mass spectrometry (SDE-GC-MS). The results showed that the palatability of dog food 2 and 3 was better than that of dog food 1. By GC-MS, 212 compounds were isolated, and 73, 95 and 103 compounds were found in dog food, respectively. The aromatic substances of dog food 1 were palmitic acid, trans-oleic acid, 10-cis-heptadecenoic acid, heptadecane, 2,6,10,14-tetramethylninethane, lauric acid, myristic acid, stearic acid, stearic acid and butylbenzyl alcohol, the aromatic substances of dog food 2 were palmitic acid, lauric acid, linoleic acid, octadecenoic acid, n-heptadecanoic acid, heptadecenoic acid, myritic acid and 2,6,10,14-tetramethylpentadecanoic acid, and the aromatic substances of dog food 3 were palmitic acid, myristic acid, palmitic acid, trans-oleic acid, n-decanoic acid, stearic acid and octadecaldehyde. The study indicates that compared with traditional chicken meal and fish oil, the dog food made from black horsefly protein powder and black horsefly oil as main animal protein and oil sources has better palatability and unique flavor composition.