化学
脱氧核酶
基因型
丙型肝炎病毒
催化作用
病毒
病毒学
甲型肝炎病毒
检出限
生物化学
色谱法
基因
生物
作者
Yuting Xu,Li Li Li,Yongrong Yang,Chengzhi Huang,Hong Yan Zou
出处
期刊:Talanta
[Elsevier]
日期:2024-05-01
卷期号:271: 125754-125754
标识
DOI:10.1016/j.talanta.2024.125754
摘要
Developing a simple, reliable, and sensitive hepatitis C virus (HCV) genetic sensing platform is of great significance for diagnosing diseases and selecting appropriate antiviral treatments. Herein, a tandem nucleic acid amplification strategy for sensitive detection of HCV genotype 1b (HCV-1b) was developed by stringing the catalytic hairpin assembly (CHA) and the triggered DNAzyme amplifier. The hairpin reactants were initiated by the target to produce lots of triggering double-stranded DNA sequences which can efficiently activate the subsequent blocked DNAzyme. Thereby, the continuous cleavage of substrate was realized, resulting in the fluorescence signal amplification. The DNA-based isothermal CHA-DNAzyme (CDz) sensing platform was successfully applied for sensitive detection of HCV-1b with the limit of detection (84 pM) and showed good selectivity. Moreover, the practical detection of target DNA in the complex biologic matrix indicated that the developing strategy had good potential for early HCV infection diagnosis.
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