First detection of Morganella morganii subsp. morganii A from diarrhea patients of army soldiers in Yunnan.

Objective To survey the infection status of Morganella morganii in PLA soldiers,identify the the biogroup A of Morganella morganii subsp.morganii,detect the endotoxin,conduct pathogenic research and test their sensitivity to antibiotics.Methods The bacteria were cultured and isolated on MacConkey gel plate,then the bacterial spepecis were identified by sysemic biochemical test.The morphology of the bacteria was observed under microscope and their endotoxins were detected by shelate test.In addition,the pathogenesis of the bacteria were investigated by injection of thebacteria into peritonea of mice and drug sensitivity test of the bacteria to antibiotics were also carried out and the results were anlayzed.Totally 7 strains of biogroup A of Morganella morganii subsp.morganii were isolated from 268 samples of diarrhea with a detection rate of 2.61%.The results of detection of endotoxin were all positive.Pathogenesis test confirmed that the bacterium was strongly toxic.Thebacterium was sensitive only to 2 antibiotics out of the 17 antibiotics tested.Conlusion The newly isolated 7 strains of biogroup A of Morganella morganii subsp.morganii,can produce endotoxins and highly toxic,the mice were died after infected.The bacerium is also resistant to most antibiotics tested.