Hemosiderin. Iron Extraction Studies.,

1. It has been demonstrated that iron is extracted from horse spleen hemosiderin at different rates by disodium ethylenediamine tetraacetate. During the first 24 hours of extraction, between 21 and 70% of total hemosiderin iron was removed by chelating agent from 8 hemosiderin preparations which contained between 33.5 and 19% iron, respectively. The amount of iron removed is inversely proportional to total hemosiderin iron concentration. Subsequently, iron is dissolved at a slow rate until practically all is removed during 25 days extraction. 2. Analytical data for amount of ferritin present in hemosiderin are presented.