Synthesis and identification of artificial antigen for Medroxyprogesterone Acetate

【Objective】 This study was to develop a rapid and sensitive technology for the detection of Medroxyprogesterone Acetate(MPA).The atrificial antigen of MPA was synthesized and identified in the study.【Method】 MPA hemi hemi-succinate(MPA-HS) was produced by the oximation reaction of MPA with the reaction between oximinooximated-MPA and with succinic anhydride,then the hapten MPA-HS was conjugated to protein carrier BSA by using the coupling agent diisopropylcarbodiimide,and the synthesis synthesized antigen MPA-BSA was identified by full-wavelength the ultraviolet full wavelength scanner,SDS-PAGE,ELISA test and animal-inoculation immunization test.【Result】 The results showed that the synthesis synthesized antigen MPA-BSA with good immunogenicity and reactogenicityantigenicity,and the optimized conjugation molecular ratio of coupling reaction was determined to be 8∶1.【Conclusion】 The synthesis of artificial antigen MPA-BSA set basis for the study of the immunology detection technology of MPA.