Electrostatic-Driven Nucleobase Discrimination by Covalent Organic Framework Nanosheets for Deoxyribonucleic Acid Methylation Profiling

) gene methylation analysis, yielding results highly consistent with gold standard pyrosequencing. This work is the first to exploit COF for direct nucleobase discrimination and quantitative DNA methylation analysis, elucidating the COF-nucleobase interaction mechanism and highlighting their potential for epigenetic detection.