Establishment of Rat Hepatic Fibrosis Model Induced by Thioacetamide

Objective: To provide suitable clinical monitoring method and theory by establishing the stable and efficient rat hepatic fibrosis model under the induction of thioacetamide (TAA), and by analyzing hepatic pathology classification and serum test index of rat .Methods: 48 SD male rats were selected and randomly divided into 4 groups, i.e. control group (12), model-Ⅰ group(12), model-Ⅱ group(12) and model-Ⅲgroup(12). According to the rat weight, using TAA intraperitoneal injection to induce hepatic fibrosis model. Drugs were injected according to the time of experiment, test serum concentrations of AST and ALT in different time periods and the histopathology examination of hepar was conducted. Results: Mortality of model -Ⅰ was 25.00% (3/12), the rate of hepatic fibrosis is 75.00%(9/12); mortality of model - Ⅱis 8.33%(1/12) and the rate of hepatic fibrosis was 91.67% (11/12); the rate of hepatic fibrosis of model - Ⅲwas 83.33%(10/12). Serum concentrations of AST and ALT increased in all group, and the liver lesions were found in liver pathological and histological examination. Conclusion: This histopathology result is reliable and simple to operate, and it is suitable for preparation of experimental hepatic fibrosis model .