下调和上调
微泡
癌症研究
基因沉默
小RNA
转移
癌症
生物
化学
生物化学
遗传学
基因
作者
Jun Lü,Yaohui Wang,Changhwan Yoon,Xiaoyan Huang,Yu Xu,Jian‐Wei Xie,Jia-Bin Wang,Lin Ji,Qi‐Yue Chen,Long‐Long Cao,Chao‐Hui Zheng,Ping Li,Chang‐Ming Huang
标识
DOI:10.1016/j.canlet.2019.11.038
摘要
The biological functions of circular RNAs (circRNAs) in gastric cancer (GC) remain largely unexplored. Here, we identified that circ-RanGAP1 was significantly upregulated in both GC tissues and exosomes from the plasma of GC patients. High circ-RanGAP1 expression was closely associated with an advanced TNM stage, lymph node metastases, and worse survival. Inhibition of circ-RanGAP1 decreased GC cell invasion and migration in vitro. Overexpression of circ-RanGAP1 had the opposite effect. Additionally, circ-RanGAP1 silencing remarkably suppressed tumor growth and metastasis of GC in vivo. Mechanistically, circ-RanGAP1 sponged miR-877-3p to upregulate VEGFA expression. Overexpression of miR-877-3p reversed the biological functions mediated by circ-RanGAP1 in GC cells. Interestingly, we demonstrated that circ-RanGAP1 was upregulated in plasma exosomes from preoperative GC patients. More importantly, the plasma exosomes derived from these patients enhanced the migration and invasion potential of GC cells. Overall, the circ-RanGAP1-mediated miR-877-3p/VEGFA axis promotes GC progression. Our findings suggest that circ-RanGAP1 might act as a potential prognostic biomarker and therapeutic target for GC treatment.
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