Novel Perylene Diimide–Benzothaizole Hybrid: A Reaction‐Based Probe for the Detection and Discrimination of H 2 S, Cys and DTT

ABSTRACT The reaction‐based probe perylene diimide–hydroxyphenyl benzothiazole ( PR ) can be used for the detection and discrimination of H 2 S, DTT and Cys in 20% HEPES buffer–DMSO and DMSO. The H 2 S induced radical anion formation of PR in 20% HEPES buffer and thiolysis of the ether bond of PR in DMSO. However, the addition of DTT showed only a decrease in the absorbance intensity and Cys showed insignificant behaviour towards PR in DMSO. The optical, AFM and DLS studies along with isolation of the reaction product in the model reaction support the interaction of the PR with bio thiols.