平滑
异三聚体G蛋白
修补
刺猬
细胞生物学
刺猬信号通路
G蛋白偶联受体
G蛋白
化学
生物
信号转导
出处
期刊:Methods in molecular biology
日期:2021-09-26
卷期号:: 205-212
标识
DOI:10.1007/978-1-0716-1701-4_18
摘要
The oncoprotein Smoothened (SMO) can transduce the Hedgehog signal from the tumor suppressor Patched-1 (PTCH1) to glioma-associated-oncogene (Gli) transcription factors. Previous studies have shown that SMO is a G-protein-coupled receptor (GPCR) of the Frizzled-class (class-F) that can activate Gi family of heterotrimeric G proteins. Here, we describe [35S]-GTPγS assay using SMO cell membranes and purified Gi protein to measure the level of Gi protein activation following the activation of SMO by agonists.
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