苯丙素
肉桂醇脱氢酶
基因
互补DNA
苯丙氨酸解氨酶
生物
锌指
生物化学
烟草
转基因
分子生物学
生物合成
氨基酸
转录因子
苯丙氨酸
作者
Akiyoshi Kawaoka,Pulla Kaothien,Kazuya Yoshida,Saori Endo,Kazutaka Yamada,Hiroyasu Ebinuma
出处
期刊:Plant Journal
[Wiley]
日期:2000-05-01
卷期号:22 (4): 289-301
被引量:166
标识
DOI:10.1046/j.1365-313x.2000.00737.x
摘要
Summary The AC‐rich motif, Pal‐box, is an important cis ‐acting element for gene expression involved in phenylpropanoid biosynthesis. A cDNA clone ( Ntlim1 ) encoding a Pal‐box binding protein was isolated by Southwestern screening. The deduced amino acid sequence is highly similar to the members of the LIM protein family that contain a zinc finger motif. Moreover, Ntlim1 had a specific DNA binding ability and transiently activated the transcription of a β‐glucuronidase reporter gene driven by the Pal‐box sequence in tobacco protoplasts. The transgenic tobacco plants with antisense Ntlim1 showed low levels of transcripts from some key phenylpropanoid pathway genes such as phenylalanine ammonia‐lyase, hydroxycinnamate CoA ligase and cinnamyl alcohol dehydrogenase. Furthermore, a 27% reduction of lignin content was observed in the transgenic tobacco with antisense Ntlim1 .
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