Surface modification of HSA containing magnetic PLGA nanoparticles by poloxamer to decrease plasma protein adsorption

Lifetime prolongation for hydrophobic drug carriers has been the focus of interest for many years. Poloxamer (Pluronic F68, PF68) has been employed in this study for modifying the surface of magnetic poly(d,l-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with human serum albumin (HSA) model drug. Surface characteristics of untreated and PF68 treated NPs were analyzed by size, zeta potential and electrophoretic mobility studies. UV-vis spectroscopic analysis, isothermal titration calorimetry (ITC) and dynamic light scattering methods were used to investigate serum protein (bovine serum albumin, BSA) adsorption. Results showed the successful surface attachment of PF68. Among different concentrations (0.1-1%, wt/vol) of PF68 studied, 0.5% was found to be the most useful, since a higher concentration can issue in micelle formation. 50% less BSA tended to be adsorbed on the treated NPs in comparison to the untreated ones.