Isolation of trans-anethole degrading bacterial strains and biotransformation of trans-anethole

Anise oil is a natural spice in Guangxi.Isolation of trans-anethole degrading bacterial strains and trans-anethole biotransformation process were performed using anise oil as the only carbon source.The target strains were expected to produce high value-added nature spice and medicine intermediate by biotransformation of trans-anethole—the main constituent of anise oil.Bacteria were isolated from soil under aniseed trees by enrichment culture.Then biotransformation of trans-anethole to anisaldehyde or anisic acid with these strains were carried out by fermentation.The contents of trans-anethole,anisaldehyde and anisic acid in the culture broth were analyzed by the methods of TLC,photometry and HPLC determined.The isolated bacterial strain BT-13 had good capability to biotransform trans-anethole to anisic acid.This strain was identified as Pseudomonas sp.according to visual observation,physiological and biochemical experiments and 16S rDNA sequence analysis.